5% Quicker W/ 135ml of Red Blood Cells - Transfusion Works Wilean 2h! Plus: ~1.5g/kg = Optimal PWO Protein Intake for Protein Synthesis in Females - SV November Short Uniques

You may remember from the SuppVersity contemporarys on Facebook that the use of cobalt supplements can indeed (as it has long been touted and doubted) have similar effects as EPO (Hoffmeister 2018). Yet while the study by Hoffmeister et al. showed that the VO2max correlated signwhethericantly with the 2% increase in Hemoglobin in response to the ingestion of  5 mg of ionized Co2+ for 3 weeks, an acute performance-enhancing effect was neither tested nor observed in the German study.

That's in contrast to autologous (=your own) blood infusions. A simple injection of only ~135 ml of red blood cells that were precedingly lonely from 450 ml of your own blood 2h before training or competition will improve your endurance performance by 5%, ... according to the latest study from the University of Copenhagen (Bejder 2018).

And whether you're rather into lwhetherting weights than cycling, running, or triathlons, nowadays's research update also discusses the results of a recent small-scale study by Malovany et al. (2018), which puts a(t least a preliminary) number on the usefulness of escalating post-workout protein intakes from tall to very tall. Sounds interesting? Well, then let's start with the "blood doping" study by Bejder, et al.

+ + + The "blood doping study" + + +

• Instant (2h post) 5% performance boost in athletes w/ 135 ml of red blood cells (#RBCs) from a single 450 ml phlebotomy (Bejder 2018) -- While you all will have heard about "blood doping" and the infusion of autologous (=their own) blood and/or lonely red blood cells (#RBCs) in cyclists and other endurance athletes, I bet that you will be as surprised as I was that a single injection of 135 ml of red blood cells (#RBC) from a rather small amount of your own blood (450 ml) will improve your endurance performance by 5% - and that only 2h after you injected the RBCs.

• Study type: Randomized, double-blind, placebo-controlled crossover study
• Comparison: placebo = sham phlebotomized vs. blood transfusion-trial
• Subjects: N=9 (=small scale) young men w/ >1year resistance training (all major muscle groups | training at least twice a week) experience who didn't do more than 30 minutes "cardio" per exercise session and were able to lwhethere 0.7x and 2.3x their bodyweight on the bench press & leg press, respectively; no supplement use, general health, you know it...
• Outcomes: Hb, Hct, RBC, and Ret% 1wk before phlebotomy, 3 days before transfusion, 2 h post 135 ml transfusion and 3 days post 135+235 ml transfusion
• Carry outance tests: (1)Two 650kcal time-trial three days and 2h after receiving either ~50% (135 ml) of the RBCs or a sham transfusion on subsequent days + (2) One 4×30 s all-out cycling sprint interspersed by 4 min of recovery was performed six days before and three days after a moment autologous blood transfusion (235 ml).

• What the scientists found was that the subjects' mean power in the time trial (TT) to was increased in time trials from before to after transfusion (P<0.05) in BT (213±35 vs. 223±38 W; mean±SD) but not in PLA (223±42 vs. 224±46 W | see Figure 1, left).
  

  Figure 1: Mean power in the TT and during the 4x30s sprints in the active and placebo trial (Bejder 2018).

  For the four 30 s sprint bouts that were performed not 2h, but three days later no effect was observed[BT(639±35vs644±26W)andPLA(638±43vs639±25W)|see[BT(639±35vs644±26W)andPLA(638±43vs639±25W)|see[BT(639±35vs644±26W)andPLA(638±43vs639±25W)|see[BT(639±35vs644±26W)andPLA(638±43vs639±25W)|seeFigure 1, right].

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What does the "blood doping"-study tell us? The study shows that there is an instant increase in endurance performance that's virtually tallly relevant for athletes competing in various sports from a relatively small amount of RBCs (~135 ml) you can additionalct from a single phlebotomy.

Improvements in sprint/anaerobic performance, on the other hand, seem unlikely. After all, despite a moment (larger = 235ml) injection of RBCs the subjects' performance during the 4x30s bouts of all-out cycling on day 3 after the initial transfusion was not increased - in the presence of elevated RBCs, that is (5.03 ± 0.28 versus 4.58 ± 0.17x10^12/L blood) | Comment!.

+ + + The "protein ceiling" study + + +

• Optimal post-workout protein intake for max. protein synthesis over 8h in resistance-trained women is 1.5-2.0g/kg - More will just  be burned as fuel (Malovany 2018) -- For men we've known this all along: There's a threshancient value not to how much protein we can in- and digest in one serving, but rather to the amount of protein that can be used to fuel skeletal muscle protein synthesis after workouts (Humayun 2007; Jäger 2017). And while they certainly have to be considered preliminary due to the small sample size of N=9, Malovany et al.'s (2018) latest study shows that a similar threshancient exists for female resistance trainees, too.

• Study type: randomized sequential study of the effect of dwhetherferent amounts of PWO protein ranging from deficient to excess (0.2-2.90 g/kg)
• Subjects: N = 8 healthy females w/ regular menstrual cycles, who perform wgap-body resistance exercise regularly, had trained each major muscle group (i.e., chest, back, and legs) at least twice per week consistently for at least one year prior to recruitment, didn't do more than 30 min of continual endurance training per exercise session, and whose 1RM on the bench press and leg press of 0.7 and 2.3 × bodyweight, respectively.
• Outcomes: steady state wgap body phenylalanine rate of appearance (Ra), oxidation (Ox; the reciprocal of protein synthesis, PS) and net protein balance protein synthesis degreed via minimally-invasive indicator amino acid oxidation (IAAO) technique (protein synthesis) and urinary Urea: Creatinine ratio (protein oxidation), respectively
• Exercise standardization: 2d prior to each metabolic trial, all participants were required to total a prescribed but self-monitored wgap-body resistance exercise session - the workout was identical to the workout they would do on the test day, i.e. i) barbell bench press, lat pulldown superset; ii) standing overhead barbell press and seated cable row superset; iii) leg press; and iv) leg extension; each exercise was performed at 75% 1RM for 4 sets of 8-10 repetitions with ~90s rest intervals between sets
• Dietary standardization: 2-d adaptation diet containing 1.2 g/kg/d of protein during the 2-d controlled training period prior to each metabolic trial
• Testing days: ingestion of liquid carbohydrate beverage (1 g/kg as a 1:1 ratio of maltodextrin: Gatorade® Terminateurance) 1h before std. workout (see exercise standardization); each participant totald 6-7 metabolic trials (n = 50 for total trials totald) during the luteal phase, which was defined as the moment half of the menstrual cycle.
• Supplementation: 8x hourly meals providing a randomized test protein intake (0.2-2.9 g/kg) as crystalline amino acids, modeled after egg protein, with fixed phenylalanine (30.5 mg/kg) and excess tyrosine (40.0 mg/kg)

• As you can see in Figure 2 (left), the scientists observed a ceiling effect for the protein synthesis with protein intakes >1.5g/kg body weight - the horizontal nature of the linear trend for the values clearly indicates this.
  
  
  The rate of protein oxidation, which was estimated via the urinary Urea: Creatinine ratio, on the other hand, increased linearly with increasing protein intakes (Figure 2, right) - without a threshancient effect, i.e. the more protein, the more is going to be oxidized (see bottom line for discussion of what would happen w/ bolus vs. 8x1/h ingestion).

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What does the "protein ceiling"-study tell us? Due to its small scale (N=9) and very specwhetheric participant characteristics (young, healthy, resistant-trained women), the results of the study at hand have to be considered as good, but preliminary evidence for the existence and quantity of a protein synthesis threshancient (~1.5g/kg/8h) during the first 8h after a workout.

In that, it is important to point out that this effect was achieved by the sequential ingestion of a total amount of 0.2-2.9 g/kg of protein every hour. It is not just likely, but nearly certain that taking the ingestion of a single bolus of the amino acid mix instantly after the workout (for 1.5g/kg that would have been 100g per subject) would have yielded dwhetherferent results. More specwhetherically, one could speculate that the protein oxidation would have increased, while the net protein synthesis would have decreased w/ dose-escalating bolus ingestion (compared to the sequential ingestion protocol used in the study at hand) - a result I've reported for whey in a 2013 article from the archives.

Speaking of whey: It is not clear to which extent the results can be generalized to dwhetherferent protein sources, like whey (concentrate, isolate, hydrolysate), micellar casein, whey+casein mixes, actual egg protein, etc. as both, their digestion kinetics, as well as their amino acid and functional peptide content dwhetherfer signwhethericantly. Still, the recommendation to consume 1.5-2.0g/kg protein wilean the 8h window after a workout to optimize protein synthesis in healthy, resistance-trained young women is the best we have as far as "one-size-fits-'em-all" recommendations are concerned | Comment!

References:

• Bejder, J. "Time Trial Carry outance Is Sensitive to Low-Volume Autologous Blood Transfusion." Medicine & Science in Sports & Exercise: November 6, 2018 - Volume Publish Ahead of Print
• Hoffmeister, Torben, et al. "Effects of 3 weeks of verbal low-dose Cobalt on hemoglobin mass and aerobic performance." Frontiers in physiology 9 (2018).
• Humayun, Mohamcrazy A., et al. "Reevaluation of the protein requirement in young men with the indicator amino acid oxidation technique–." The American journal of clinical nutrition 86.4 (2007): 995-1002.
• Jäger, Ralf, et al. "International society of sports nutrition position stand: protein and exercise." Journal of the International Society of Sports Nutrition 14.1 (2017): 20.
• Malowany, Julia M., et al. "Protein to Maximize Wgap-Body Anabolism in Resistance-trained Females after Exercise." Medicine & Science in Sports & Exercise (2018).

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